pAAV2-CAG-3xNLS-AausFP1
(Plasmid #191096)

• Purpose: To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
• Depositing Labs: Murray Blackmore, Pantelis Tsoulfas
• Publication: Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi: 10.7554/eLife.76254.

Backbone

• Vector backbone: pAAV2-CAG
• Backbone manufacturer: Original was from the Boyden laboratory
• Backbone size w/o insert (bp): 4629
• Total vector size (bp): 5430
• Vector type: Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3xNLS-AausFP1
• Species: Synthetic
• Insert Size (bp): 801
• Mutation: Optimized to Human codon usage
• Promoter: CMV enhancer and CAG
• Tag / Fusion Protein:
  • Three repeats of the nuclear localizzation sequences from SV40 fused to Aequorea . cf. australis FP 1. FPbase ID: C2FJQ (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pCAGG-5
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry