pAAV2-CAG-mGreenLantern-KASH
(Plasmid #191094)

• Purpose: To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
• Depositing Labs: Murray Blackmore, Pantelis Tsoulfas
• Publication: Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi: 10.7554/eLife.76254.

Backbone

• Vector backbone: pAAV2-CAG
• Backbone size w/o insert (bp): 4794
• Total vector size (bp): 5777
• Vector type: Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mGreenLantern-KASH
• Insert Size (bp): 983
• Mutation: Optimizzation was done using the Human codon usage
• Promoter: CMV enhancer and CAG
• Tag / Fusion Protein:
  • mGreenLantern fused to the KASH domain of Nesprin 2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry