pEY79
(Plasmid
#191074)
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Purposeflp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191074 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPD95.62
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameflp-12
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SpeciesC. elegans (nematode)
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MutationNone
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Entrez Geneflp-12 (a.k.a. CELE_C05E11.8)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEY79 was a gift from Oliver Hobert & Eviatar Yemini (Addgene plasmid # 191074 ; http://n2t.net/addgene:191074 ; RRID:Addgene_191074) -
For your References section:
NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification in C. elegans. Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT, Paninski L, Venkatachalam V, Hobert O. Cell. 2021 Jan 7;184(1):272-288.e11. doi: 10.1016/j.cell.2020.12.012. Epub 2020 Dec 29. 10.1016/j.cell.2020.12.012 PubMed 33378642
