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pcDNA-EGFP MASTL E167D (siRNA resistant)
(Plasmid #191013)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191013 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4735
  • Total vector size (bp) 7356
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Microtubule-associated serine/threonine kinase like
  • Species
    H. sapiens (human)
  • Mutation
    E167D
  • GenBank ID
    84930
  • Entrez Gene
    MASTL (a.k.a. GREATWALL, GW, GWL, MAST-L, THC2)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-EGFP MASTL E167D (siRNA resistant) was a gift from Johanna Ivaska (Addgene plasmid # 191013 ; http://n2t.net/addgene:191013 ; RRID:Addgene_191013)

  • For your References section:

    MASTL promotes cell contractility and motility through kinase-independent signaling. Taskinen ME, Narva E, Conway JRW, Hinojosa LS, Lilla S, Mai A, De Franceschi N, Elo LL, Grosse R, Zanivan S, Norman JC, Ivaska J. J Cell Biol. 2020 Jun 1;219(6). pii: 151688. doi: 10.1083/jcb.201906204. 10.1083/jcb.201906204 PubMed 32311005
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