pLenti6.3/TO/V5-DEST-IGFBP2-Clover
(Plasmid #191008)

• Purpose: Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag
• Depositing Lab: Johanna Ivaska
• Publication: Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi: 10.1126/sciadv.adg1840. Epub 2023 Jul 12.

Backbone

• Vector backbone: pLenti6.3/TO/V5-DEST
• Backbone manufacturer: Invitrogen (Life Technologies)
• Backbone size w/o insert (bp): 9351
• Total vector size (bp): 9458
• Vector type: Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Insulin-Like Growth Factor Binding Protein 2
• Species: H. sapiens (human)
• Insert Size (bp): 1710
• GenBank ID: 3485
• Entrez Gene: IGFBP2 (a.k.a. IBP2, IGF-BP53)
• Promoter: CMV
• Tag / Fusion Protein:
  • Clover (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV_F
• 3′ sequencing primer: WPRE_R

Resource Information

• Supplemental Documents:
  • pLenti6.3-IGFBP2-Clover.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry