pBTK1010 (pSL25)
(Plasmid #191005)

• Purpose: Expresses blue chromoprotein on a broad host range RP4 origin plasmid
• Depositing Lab: Jeffrey Barrick
• Publication: Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 10.1038/s43705-023-00255-3.

Backbone

• Vector backbone: pTD-C_sYFPTwinStrep
• Backbone size w/o insert (bp): 3309
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: blue chromoprotein
• Alt name: amilCP
• Alt name: BBa_K592009
• Species: Acropora millepora
• Insert Size (bp): 663
• Promoter: CP25

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • pBTK_1010_psl25.gbk
• A portion of this plasmid was derived from a plasmid made by: Addgene, amilCP chromoprotein

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint.