pBTK1009 (pSL9)
(Plasmid
#191004)
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PurposeExpresses E2 Crimson on a broad host range pBBR1 origin plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBBR1MCS-2
- Backbone size w/o insert (bp) 4528
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameE2-Crimson
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SpeciesDiscosoma sp.
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Insert Size (bp)717
- Promoter CP25
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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A portion of this plasmid was derived from a plasmid made bypBTK570
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBTK1009 (pSL9) was a gift from Jeffrey Barrick (Addgene plasmid # 191004 ; http://n2t.net/addgene:191004 ; RRID:Addgene_191004) -
For your References section:
The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae. Elston KM, Phillips LE, Leonard SP, Young E, Holley JC, Ahsanullah T, McReynolds B, Moran NA, Barrick JE. ISME Commun. 2023 May 24;3(1):49. doi: 10.1038/s43705-023-00255-3. 10.1038/s43705-023-00255-3 PubMed 37225918
