pBTK1004 (pSL3)
(Plasmid #190999)

• Purpose: Expresses red chromoprotein on a broad host range RSF1010 origin plasmid
• Depositing Lab: Jeffrey Barrick
• Publication: Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 10.1038/s43705-023-00255-3.

Backbone

• Vector backbone: pBTK402
• Backbone size w/o insert (bp): 7023
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: red chromoprotein
• Alt name: mRFP1
• Alt name: BBa_E1010
• Species: Discosoma sp.
• Insert Size (bp): 682
• Promoter: CP25

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI-v2 (destroyed during cloning)
• 3′ cloning site: BsmBI-v2 (destroyed during cloning)

Resource Information

• Supplemental Documents:
  • pBTK1004_psl3.gbk
• A portion of this plasmid was derived from a plasmid made by: IGEM registry, Bba_E1010

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.02.15.528778v1 for bioRxiv preprint.