Dom34+GST
(Plasmid
#19099)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET28b(+)
- Backbone size w/o insert (bp) 5369
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameProtein DOM34
-
Alt nameSaccharomyces cerevisiae
-
SpeciesS. cerevisiae (budding yeast)
-
Insert Size (bp)936
-
Entrez GeneDOM34 (a.k.a. YNL001W)
-
Tags
/ Fusion Proteins
- C tag (C terminal on insert)
- N tag (N terminal on insert)
- GST
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer t7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Dom34+GST was a gift from SeWon Suh (Addgene plasmid # 19099 ; http://n2t.net/addgene:19099 ; RRID:Addgene_19099) -
For your References section:
Structural and functional insights into dom34, a key component of no-go mRNA decay. Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park CM, Suh SW. Mol Cell. 2007 Sep 21. 27(6):938-50. 10.1016/j.molcel.2007.07.019 PubMed 17889667