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pET-PLS-KP6-E67A_rpoA-U
(Plasmid #190973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PLS-KP6
  • Species
    Synthetic
  • Mutation
    DYW-KP:E67A
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-PLS-KP6-E67A_rpoA-U was a gift from Bernard Gutmann (Addgene plasmid # 190973 ; http://n2t.net/addgene:190973 ; RRID:Addgene_190973)

  • For your References section:

    U-to-C RNA editing by synthetic PPR-DYW proteins in bacteria and human culture cells. Ichinose M, Kawabata M, Akaiwa Y, Shimajiri Y, Nakamura I, Tamai T, Nakamura T, Yagi Y, Gutmann B. Commun Biol. 2022 Sep 15;5(1):968. doi: 10.1038/s42003-022-03927-3. 10.1038/s42003-022-03927-3 PubMed 36109586
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