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Addgene

hPCNA-C148S
(Plasmid #190939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3c
  • Backbone size w/o insert (bp) 4638
  • Total vector size (bp) 5421
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Proliferating Cell Nuclear Antigen
  • Alt name
    PCNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    783
  • Mutation
    changed cysteine 148 to serine
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter T7

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPCNA-C148S was a gift from Brian Kelch (Addgene plasmid # 190939 ; http://n2t.net/addgene:190939 ; RRID:Addgene_190939)

  • For your References section:

    A thermosensitive PCNA allele underlies an ataxia-telangiectasia-like disorder. Magrino J, Munford V, Martins DJ, Homma TK, Page B, Gaubitz C, Freire BL, Lerario AM, Vilar JB, Amorin A, Leao EKE, Kok F, Menck CF, Jorge AA, Kelch BA. J Biol Chem. 2023 May;299(5):104656. doi: 10.1016/j.jbc.2023.104656. Epub 2023 Mar 27. 10.1016/j.jbc.2023.104656 PubMed 36990216
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