Skip to main content
Addgene

hPCNA-S228I
(Plasmid #190938)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET3c
  • Backbone size w/o insert (bp) 4638
  • Total vector size (bp) 5421
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Proliferating Cell Nuclear Antigen
  • Alt name
    PCNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    783
  • Mutation
    changed serine 228 to isoleucine
  • GenBank ID
    NM_002592.2 NM_002592.2
  • Entrez Gene
    PCNA (a.k.a. ATLD2)
  • Promoter T7

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hPCNA-S228I was a gift from Brian Kelch (Addgene plasmid # 190938 ; http://n2t.net/addgene:190938 ; RRID:Addgene_190938)

  • For your References section:

    A Disease-Causing Variant in PCNA Disrupts a Promiscuous Protein Binding Site. Duffy CM, Hilbert BJ, Kelch BA. J Mol Biol. 2016 Mar 27;428(6):1023-1040. doi: 10.1016/j.jmb.2015.11.029. Epub 2015 Dec 11. 10.1016/j.jmb.2015.11.029 PubMed 26688547
Related items: