pCFJ782 - HygroR
(Plasmid #190933)

• Purpose: Hygromycin resistance co-injection marker
• Depositing Lab: Christian Froekjaer-Jensen
• Publication: El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184.

Backbone

• Vector backbone: pDONR1-2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2544
• Total vector size (bp): 4281
• Vector type: Worm Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Hygromycin resistance gene
• Alt name: HygroR
• Species: C. elegans (nematode)
• Promoter: rps-0

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • pCFJ782 - HygroR.gb
• A portion of this plasmid was derived from a plasmid made by: The hygromycin resistance gene was originally developed by the Chin lab (Cambridge U) in the publication Radman et al. (2013) Plos One (https://pubmed.ncbi.nlm.nih.gov/24130756/).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The plasmid can be used as a co-injection marker to select for transgenic C. elegans using hygromycin B selection.