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Addgene

pCEF-S15-FRB-Bla1
(Plasmid #190916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCEF
  • Total vector size (bp) 5545
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S15-FRB-Bla1 fusion protein
  • Species
    Synthetic
  • Insert Size (bp)
    960
  • Promoter CEF
  • Tag / Fusion Protein
    • N-terminal fragment of BLA fused to c-Src and FRB domains

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEF-S15-FRB-Bla1 was a gift from Jakob Reiser (Addgene plasmid # 190916 ; http://n2t.net/addgene:190916 ; RRID:Addgene_190916)

  • For your References section:

    Rapid titration of retroviral vectors using a beta-lactamase protein fragment complementation assay. Ou W, Marino MP, Lu C, Reiser J. Gene Ther. 2013 Jan;20(1):43-50. doi: 10.1038/gt.2011.212. Epub 2012 Jan 19. 10.1038/gt.2011.212 PubMed 22257933
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