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Addgene

pLTR-DuvSAF
(Plasmid #190914)

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Item Catalog # Description Quantity Price (USD)
Plasmid 190914 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Total vector size (bp) 5651
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lyssavirus duvenhage antigenic glycoprotein
  • Species
    Synthetic
  • Insert Size (bp)
    1602
  • GenBank ID
    AF298147
  • Promoter HIV-1 LTR

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLTR-DuvSAF was a gift from Jakob Reiser (Addgene plasmid # 190914 ; http://n2t.net/addgene:190914 ; RRID:Addgene_190914)

  • For your References section:

    Comparative analysis of HIV-1-based lentiviral vectors bearing lyssavirus glycoproteins for neuronal gene transfer. Federici T, Kutner R, Zhang XY, Kuroda H, Tordo N, Boulis NM, Reiser J. Genet Vaccines Ther. 2009 Jan 13;7:1. doi: 10.1186/1479-0556-7-1. 10.1186/1479-0556-7-1 PubMed 19144125
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