pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1
(Plasmid #190902)

• Purpose: AAV vector expressing thew GFP reporter gene and human sgHTT
• Depositing Lab: Nicole Déglon
• Publication: Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;29:254-270. doi: 10.1016/j.omtm.2023.03.013. eCollection 2023 Jun 8.

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Backbone

• Vector backbone: AAVss
• Backbone size w/o insert (bp): 2947
• Total vector size (bp): 4390
• Vector type: AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP and sgHTT
• Species: H. sapiens (human)
• Insert Size (bp): 1443
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: EFS and U6

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: GCC CAC AGT CCC CGA GAA GTT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry