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pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2
(Plasmid #190898)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190898 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMK
  • Backbone manufacturer
    Geneart
  • Backbone size (bp) 2278
  • Modifications to backbone
    gene synthesis of sgRNA expression cassette
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Promoter U6 and 7sk

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMK-AttL1-NotI/BamHI-U6-BsaI-tracrRNAopt-BamHI-U6-SapI-tracrRNAopt-NotI/XbaI-7sk-BsmBI-tracrRNA-XbaI-AttL2 was a gift from Nicole Déglon (Addgene plasmid # 190898 ; http://n2t.net/addgene:190898 ; RRID:Addgene_190898)

  • For your References section:

    Semi-automated workflows to quantify AAV transduction in various brain areas and predict gene editing outcome for neurological disorders. Duarte F, Ramosaj M, Hasanovic E, Regio S, Sipion M, Rey M, Deglon N. Mol Ther Methods Clin Dev. 2023 Mar 27;29:254-270. doi: 10.1016/j.omtm.2023.03.013. eCollection 2023 Jun 8. 10.1016/j.omtm.2023.03.013 PubMed 37090478
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