pLTR-Rabies PV
(Plasmid
#190897)
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PurposePseudotyping of lentiviral vectors
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190897 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Total vector size (bp) 5636
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLyssavirus rabies glycoprotein
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SpeciesSynthetic
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Insert Size (bp)1575
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GenBank IDA14671
- Promoter HIV-1 LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLTR-Rabies PV was a gift from Jakob Reiser (Addgene plasmid # 190897 ; http://n2t.net/addgene:190897 ; RRID:Addgene_190897) -
For your References section:
Comparative analysis of HIV-1-based lentiviral vectors bearing lyssavirus glycoproteins for neuronal gene transfer. Federici T, Kutner R, Zhang XY, Kuroda H, Tordo N, Boulis NM, Reiser J. Genet Vaccines Ther. 2009 Jan 13;7:1. doi: 10.1186/1479-0556-7-1. 10.1186/1479-0556-7-1 PubMed 19144125