pJFRC7-syt1-GCaMP6f
(Plasmid
#190896)
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PurposeGCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJFRC7-20XUAS
- Backbone size w/o insert (bp) 8200
- Total vector size (bp) 11000
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesyt1-GCaMP6f
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SpeciesD. melanogaster (fly)
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Insert Size (bp)2700
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJFRC7-syt1-GCaMP6f was a gift from Tom Clandinin (Addgene plasmid # 190896 ; http://n2t.net/addgene:190896 ; RRID:Addgene_190896) -
For your References section:
Visual and motor signatures of locomotion dynamically shape a population code for feature detection in Drosophila. Turner MH, Krieger A, Pang MM, Clandinin TR. Elife. 2022 Oct 27;11. pii: 82587. doi: 10.7554/eLife.82587. 10.7554/eLife.82587 PubMed 36300621
Map uploaded by the depositor.
