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Addgene

pCMV-SpaCas12f1-gRNA_MS13
(Plasmid #190877)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190877 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SpaCas12f1-gRNA_MS13 was a gift from Quanjiang Ji (Addgene plasmid # 190877 ; http://n2t.net/addgene:190877 ; RRID:Addgene_190877)

  • For your References section:

    Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease. Wang Y, Wang Y, Pan D, Yu H, Zhang Y, Chen W, Li F, Wu Z, Ji Q. Cell Rep. 2022 Sep 27;40(13):111418. doi: 10.1016/j.celrep.2022.111418. 10.1016/j.celrep.2022.111418 PubMed 36170834
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