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Addgene

pAAV-CAG-EGFP barcode-8-SV40 polyA
(Plasmid #190872)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190872 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-tdTomato (codon diversified)(Plasmid #59462)
  • Backbone manufacturer
    Addgene
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-barcode8
  • Species
    Other
  • Insert Size (bp)
    718
  • Mutation
    WT

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ sequencing primer BGH
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.05.16.444258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-EGFP barcode-8-SV40 polyA was a gift from Yuejun Chen (Addgene plasmid # 190872 ; http://n2t.net/addgene:190872 ; RRID:Addgene_190872)

  • For your References section:

    High-throughput mapping of single-neuron projection and molecular features by retrograde barcoded labeling. Xu P, Peng J, Yuan T, Chen Z, He H, Wu Z, Li T, Li X, Wang L, Gao L, Yan J, Wei W, Li CT, Luo ZG, Chen Y. Elife. 2024 Feb 23;13:e85419. doi: 10.7554/eLife.85419. 10.7554/eLife.85419 PubMed 38390967
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