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Addgene

Ta1098(130-242)
(Plasmid #19087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b(+)
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cell division protein pelota related protein
  • Alt name
    Thermoplasma acidophilum
  • Species
    Thermoplasma acidophilum
  • Insert Size (bp)
    339
  • Entrez Gene
    DOM34 (a.k.a. YNL001W)
  • Tags / Fusion Proteins
    • C tag (C terminal on insert)
    • N tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ta1098(130-242) was a gift from SeWon Suh (Addgene plasmid # 19087 ; http://n2t.net/addgene:19087 ; RRID:Addgene_19087)

  • For your References section:

    Structural and functional insights into dom34, a key component of no-go mRNA decay. Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park CM, Suh SW. Mol Cell. 2007 Sep 21. 27(6):938-50. 10.1016/j.molcel.2007.07.019 PubMed 17889667
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