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pLIB_BSSHI_6xHisTEV-WIPI2d
(Plasmid #190863)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190863 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLIB
  • Backbone manufacturer
    Jan-Michael Peters
  • Backbone size w/o insert (bp) 4970
  • Total vector size (bp) 6242
  • Vector type
    Bacterial Expression, Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WIPI2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1278
  • Mutation
    SNP C to G at position 4562 (Val)
  • GenBank ID
    NC_000007.14
  • Entrez Gene
    WIPI2 (a.k.a. ATG18B, Atg21, CGI-50, IDDSSA, WIPI-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BssHII (not destroyed)
  • 3′ cloning site HindII (not destroyed)
  • 5′ sequencing primer TCCCTGAAAATACAGGTTTTC
  • 3′ sequencing primer ACAAACCACAACTAGAATGCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLIB_BSSHI_6xHisTEV-WIPI2d was a gift from Sascha Martens (Addgene plasmid # 190863 ; http://n2t.net/addgene:190863 ; RRID:Addgene_190863)
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