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pGEX-4T1_ATG4B_BamHI_NotI
(Plasmid #190862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 6134
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ATG4B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1182
  • GenBank ID
    NC_000002.12
  • Entrez Gene
    ATG4B (a.k.a. APG4B, AUTL1, HsAPG4B)
  • Tag / Fusion Protein
    • GST, Thrombin Cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotHI (not destroyed)
  • 5′ sequencing primer TGGCAAGCCACGTTTGGTGG
  • 3′ sequencing primer CACCGAAACGCGCGAGGCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T1_ATG4B_BamHI_NotI was a gift from Sascha Martens (Addgene plasmid # 190862 ; http://n2t.net/addgene:190862 ; RRID:Addgene_190862)

  • For your References section:

    A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy. Fracchiolla D, Chang C, Hurley JH, Martens S. J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 10.1083/jcb.201912098. 10.1083/jcb.201912098 PubMed 32437499
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