Skip to main content
Addgene

pBIG1b_10xHisTEVATG5_ATG12
(Plasmid #190861)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBIG1b
  • Backbone manufacturer
    Jan-Michael Peters
  • Backbone size w/o insert (bp) 5915
  • Total vector size (bp) 8315
  • Vector type
    Bacterial Expression, Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATG5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    894
  • GenBank ID
    NC_000006.12
  • Entrez Gene
    ATG5 (a.k.a. APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5)
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
  • 3′ sequencing primer TCCTCTAGTACTTCTCGACAAGCTTTTAGTCCGTAGGTTGGGG
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATG12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    423
  • GenBank ID
    NC_000005.10
  • Entrez Gene
    ATG12 (a.k.a. APG12, APG12L, FBR93, HAPG12)

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
  • 3′ sequencing primer CCACCATCGGGCGCGGATCCATGGCCGAAGAGCCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBIG1b_10xHisTEVATG5_ATG12 was a gift from Sascha Martens (Addgene plasmid # 190861 ; http://n2t.net/addgene:190861 ; RRID:Addgene_190861)

  • For your References section:

    A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy. Fracchiolla D, Chang C, Hurley JH, Martens S. J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 10.1083/jcb.201912098. 10.1083/jcb.201912098 PubMed 32437499
Related items:
Commonly requested with: