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ReactionC_pBIG1a_ATG7_ATG10
(Plasmid #190860)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190860 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBIG1a
  • Backbone manufacturer
    Jan-Michael Peters
  • Backbone size w/o insert (bp) 5915
  • Total vector size (bp) 9773
  • Vector type
    Bacterial Expression, Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATG7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2112
  • GenBank ID
    NC_000003.12
  • Entrez Gene
    ATG7 (a.k.a. APG7-LIKE, APG7L, GSA7, SCAR31)

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer CCACCATCGGGCGCGGATCCATGGCAGCGGCTACTGG
  • 3′ sequencing primer TCCTCTAGTACTTCTCGACAAGCTTTTAAATAGTCTCATCGTCGCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATG10
  • Insert Size (bp)
    663
  • GenBank ID
    NC_000005.10
  • Entrez Gene
    ATG10 (a.k.a. APG10, APG10L, pp12616)

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer CCACCATCGGGCGCGGATCCATGGAGGAGGACGAGTTC
  • 3′ sequencing primer AACCCCGATTGAGATATAGATTTATTTAAATGGTTATGATAGTTATTGCTCAGCG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ReactionC_pBIG1a_ATG7_ATG10 was a gift from Sascha Martens (Addgene plasmid # 190860 ; http://n2t.net/addgene:190860 ; RRID:Addgene_190860)

  • For your References section:

    A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy. Fracchiolla D, Chang C, Hurley JH, Martens S. J Cell Biol. 2020 Jul 6;219(7). pii: 151802. doi: 10.1083/jcb.201912098. 10.1083/jcb.201912098 PubMed 32437499
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