pcDNA3.1-OGT_opt
(Plasmid #190858)

• Purpose: For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.
• Depositing Lab: Gerald Hart
• Publication: Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016.

Backbone

• Vector backbone: pcDNA3.1
• Total vector size (bp): 8554
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: O-GlcNAc Transferase
• Species: H. sapiens (human)
• Insert Size (bp): 3144
• Mutation: Codon optimized for expression in human cells.
• Entrez Gene: OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F, T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • 190858_pcDNA3.1-OGT_opt.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA