pSilencer2.1-U6-3JB8R+12
(Plasmid #190855)

• Purpose: For mammalian expression of 3JB8R+12, a dual-specificity aptamer that binds OGT and β-catenin. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
• Depositing Lab: Gerald Hart
• Publication: Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016.

Backbone

• Vector backbone: pSilencer2.1-U6 hygro
• Total vector size (bp): 5177
• Vector type: Mammalian Expression, Synthetic Biology, Affinity Reagent/ Antibody
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3JB8R_bc339+12bp (A Dual-Specificity aptamer targeting OGT and β-catenin)
• Species: Synthetic
• Insert Size (bp): 333
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7, LKO.1 5'
• 3′ sequencing primer: 2.0Rev

Resource Information

• Supplemental Documents:
  • 190855_pSilencer2.1-U6-3JB8R+12.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA