pSilencer2.1-U6-3JB1R+4
(Plasmid #190841)

• Purpose: For mammalian expression of 3JB1R+4, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
• Depositing Lab: Gerald Hart
• Publication: Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016.

Backbone

• Vector backbone: pSilencer2.1-U6 hygro
• Total vector size (bp): 5141
• Vector type: Mammalian Expression, Synthetic Biology, Affinity Reagent/ Antibody
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3JB1R_AP3+4bp (A Dual-Specificity aptamer targeting OGT and GFP)
• Species: Synthetic
• Insert Size (bp): 303
• Promoter: U6

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7, LKO.1 5'
• 3′ sequencing primer: 2.0Rev

Resource Information

• Supplemental Documents:
  • 190841_pSilencer2.1-U6-3JB1R+4.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Sequence of the AP3 portion is from the article below:
Shui, B., Ozer, A., Zipfel, W., Sahu, N., Singh, A., Lis, J.T., Shi, H., and Kotlikoff, M.I. (2012). RNA aptamers that functionally interact with green fluorescent protein and its derivatives. Nucleic Acids Res 40, e39. 10.1093/nar/gkr1264.