Skip to main content
Addgene

pSilencer2.1-U6-T1-U6-AP3
(Plasmid #190828)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 190828 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pSilencer2.1-U6 hygro
  • Total vector size (bp) 5290
  • Vector type
    Mammalian Expression, Synthetic Biology, Affinity Reagent/ Antibody
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    T1 (an RNA aptamer of OGT)
  • Species
    Synthetic
  • Insert Size (bp)
    98
  • Promoter U6

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    AP3 (an RNA aptamer of GFP)
  • Species
    Synthetic
  • Insert Size (bp)
    84
  • Promoter U6

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequence of the AP3 portion is from the article below:
Shui, B., Ozer, A., Zipfel, W., Sahu, N., Singh, A., Lis, J.T., Shi, H., and Kotlikoff, M.I. (2012). RNA aptamers that functionally interact with green fluorescent protein and its derivatives. Nucleic Acids Res 40, e39. 10.1093/nar/gkr1264.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSilencer2.1-U6-T1-U6-AP3 was a gift from Gerald Hart (Addgene plasmid # 190828 ; http://n2t.net/addgene:190828 ; RRID:Addgene_190828)
  • For your References section:

    Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on beta-catenin. Zhu Y, Hart GW. Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016. 10.1016/j.cell.2022.12.016 PubMed 36626902