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Addgene

pEGFP-β-catenin_S23A,T40A,T41A,T112A
(Plasmid #190824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Total vector size (bp) 7362
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    β-catenin
  • Alt name
    CTNNB1
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2343
  • Mutation
    S23A, T40A, T41A, T112A
  • GenBank ID
  • Entrez Gene
    CTNNB1 (a.k.a. CHBCAT)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-β-catenin_S23A,T40A,T41A,T112A was a gift from Gerald Hart (Addgene plasmid # 190824 ; http://n2t.net/addgene:190824 ; RRID:Addgene_190824)

  • For your References section:

    Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on beta-catenin. Zhu Y, Hart GW. Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016. 10.1016/j.cell.2022.12.016 PubMed 36626902
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