pET24a-ncOGT-TPR
(Plasmid #190822)

• Purpose: For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
• Depositing Lab: Gerald Hart
• Publication: Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016.

Backbone

• Vector backbone: pET24a
• Total vector size (bp): 8345
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: O-GlcNAc Transferase
• Species: H. sapiens (human)
• Insert Size (bp): 3105
• Mutation: C467X. Also codon optimized for expression in E.coli.
• Entrez Gene: OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Supplemental Documents:
  • Plasmid 190822.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA