pET24a-ncOGT-FL
(Plasmid
#190821)
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PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET24a
- Total vector size (bp) 8345
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameO-GlcNAc Transferase
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SpeciesH. sapiens (human)
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Insert Size (bp)3105
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MutationCodon optimized for expression in E.coli
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Entrez GeneOGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
- Promoter T7
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Tag
/ Fusion Protein
- 6x His (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24a-ncOGT-FL was a gift from Gerald Hart (Addgene plasmid # 190821 ; http://n2t.net/addgene:190821 ; RRID:Addgene_190821) -
For your References section:
Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on beta-catenin. Zhu Y, Hart GW. Cell. 2022 Dec 30:S0092-8674(22)01529-X. doi: 10.1016/j.cell.2022.12.016. 10.1016/j.cell.2022.12.016 PubMed 36626902
Map uploaded by the depositor.
