pCfuNca-MCS
(Plasmid
#190820)
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PurposeMultiple cloning site between Nitzschia captiva promoter and termination regions
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescriptII SK+
- Backbone size w/o insert (bp) 2920
- Total vector size (bp) 7485
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Vector typeBacterial Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameMultiple cloning site with SpeI and PstI
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Insert Size (bp)12
Gene/Insert 2
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Gene/Insert nameBleomycin resistance gene
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Alt nameble
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Insert Size (bp)375
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Nicole Poulsen and Dr. Nils Kröger created the Cylindrotheca ble gene part of this vector
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCfuNca-MCS was a gift from Peter Kroth (Addgene plasmid # 190820 ; http://n2t.net/addgene:190820 ; RRID:Addgene_190820) -
For your References section:
Genetic transformation methods for diatom Nitzschia captiva: New tools to better understand dinotom endosymbiosis. Sprecher BN, Buck JM, Ropella LL, Ramsperger A, Kroth PG, Yamada N. Algal Research. 2023 May; Vol 72: 103136. doi: 10.1016/j.algal.2023.103136 10.1016/j.algal.2023.103136