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Addgene

pCfuNca-Mito
(Plasmid #190819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescriptII SK+
  • Backbone size w/o insert (bp) 2920
  • Total vector size (bp) 7485
  • Vector type
    Bacterial Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Diatom mitochondrial targeted GFP
  • Alt name
    GFP
  • Insert Size (bp)
    882

Gene/Insert 2

  • Gene/Insert name
    Bleomycin resistance gene
  • Alt name
    ble
  • Insert Size (bp)
    375

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Nicole Poulsen and Dr. Nils Kröger created the Cylindrotheca ble gene part of this vector

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfuNca-Mito was a gift from Peter Kroth (Addgene plasmid # 190819 ; http://n2t.net/addgene:190819 ; RRID:Addgene_190819)

  • For your References section:

    Genetic transformation methods for diatom Nitzschia captiva: New tools to better understand dinotom endosymbiosis. Sprecher BN, Buck JM, Ropella LL, Ramsperger A, Kroth PG, Yamada N. Algal Research. 2023 May; Vol 72: 103136. doi: 10.1016/j.algal.2023.103136 10.1016/j.algal.2023.103136
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