pTet-O-APOE-E3-T2A-Puro
(Plasmid
#190812)
-
PurposeExpress APOE E3 allele from tet-inducible promoter, co-expressing Puro resistance gene
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190812 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepTet-O
- Backbone size w/o insert (bp) 8395
-
Vector typeLentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAPOE
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1618
-
MutationE3
-
Entrez GeneAPOE (a.k.a. AD2, APO-E, ApoE4, LDLCQ5, LPG)
- Promoter Tet-O
Cloning Information
- Cloning method Gibson Cloning
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
E3: rs429358-T and rs7412-C
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTet-O-APOE-E3-T2A-Puro was a gift from Ronald Hart (Addgene plasmid # 190812 ; http://n2t.net/addgene:190812 ; RRID:Addgene_190812)
Map uploaded by the depositor.
