FUGW-APPIce-T2A-mCherry
(Plasmid #190806)

• Purpose: Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry
• Depositing Lab: Ronald Hart
• Publication: Hart Lab APP and APOE Plasmids 2022 (unpublished)

Backbone

• Vector backbone: FUGW
• Backbone size w/o insert (bp): 9210
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: APP
• Species: H. sapiens (human)
• Insert Size (bp): 3108
• Mutation: Icelandic (A673T)
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: Ubi C

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • 190806 FUGW-APPIce-T2A-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human APP coding sequence is from NM_201414, APP variant 3.

APP Protective (Icelandic) mutation: A673T. Reference Assembly: GRCh37 (105)/Position: Chr21:27269932 G>A. dbSNP ID: rs63750847. Codon Change: GCA to ACA.