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FUGW-APPSwe-T2A-Hygro
(Plasmid #190805)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190805 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW
  • Backbone size w/o insert (bp) 9199
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    APP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3396
  • Mutation
    Swedish (KM670/671NL)
  • Entrez Gene
    APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
  • Promoter Ubi C

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The human APP coding sequence is from NM_201414, APP variant 3.

APP Swedish mutation: KM670/671NL. Reference Assembly: GRCh37 (105)/Position: Chr21:27269939 G>T, Chr21:27269938 A>C. dbSNP ID: rs63751263, rs63750445. Codon Change: AAG.ATG to AAT.CTG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUGW-APPSwe-T2A-Hygro was a gift from Ronald Hart (Addgene plasmid # 190805 ; http://n2t.net/addgene:190805 ; RRID:Addgene_190805)
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