FUGW-APPSwe-T2A-mCherry
(Plasmid #190804)

• Purpose: Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry
• Depositing Lab: Ronald Hart
• Publication: Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844. doi: 10.1523/JNEUROSCI.0172-22.2023. Epub 2023 Jan 30.

Backbone

• Vector backbone: FUGW
• Backbone size w/o insert (bp): 9210
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: APP
• Species: H. sapiens (human)
• Insert Size (bp): 3108
• Mutation: Swedish (KM670/671NL)
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
• Promoter: Ubi C

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • 190804 FUGW-APPSwe-T2A-mCherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human APP coding sequence is from NM_201414, APP variant 3.

APP Swedish mutation: KM670/671NL. Reference Assembly: GRCh37 (105)/Position: Chr21:27269939 G>T, Chr21:27269938 A>C. dbSNP ID: rs63751263, rs63750445. Codon Change: AAG.ATG to AAT.CTG