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Addgene

PRE-Hsp70BbCas9_1.3
(Plasmid #190797)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190797 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AttB with mini-white
  • Total vector size (bp) 16870
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Follow the same growth instructions as indicated for Addgene plasmid #122486
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
  • Species
    D. melanogaster (fly)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Depositor recommends running a restriction digest to verify the correct size of the poly PREs, as indicated in the “Cloning and storage of PRExpress plasmid" document for Addgene plasmid #122486: https://www.addgene.org/122486/.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRE-Hsp70BbCas9_1.3 was a gift from Omar Akbari (Addgene plasmid # 190797 ; http://n2t.net/addgene:190797 ; RRID:Addgene_190797)

  • For your References section:

    Polycomb response elements reduce leaky expression of Cas9 under temperature-inducible Hsp70Bb promoter in Drosophila melanogaster. Warsinger-Pepe N, Chang C, Desroberts CR, Akbari OS. G3 (Bethesda). 2023 Apr 11;13(4):jkad024. doi: 10.1093/g3journal/jkad024. 10.1093/g3journal/jkad024 PubMed 36705519
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