pUB6-HuMGF
(Plasmid #190782)

• Purpose: Expresses membrane-bound form of human SCF in mammalian cells
• Depositing Lab: Jakob Reiser
• Publication: Zhang et al Retrovirology. 2010 Jan 25;7:3. doi: 10.1186/1742-4690-7-3.

Backbone

• Vector backbone: pUB6/V5-HisB
• Total vector size (bp): 5327
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human stem cell factor
• Alt name: KIT ligand
• Species: H. sapiens (human)
• Entrez Gene: KITLG (a.k.a. DCUA, DFNA69, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, SLF, WS2F)
• Promoter: UbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7 Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Paul Schwarzenberger

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry