pUB6-VSV-GS
(Plasmid
#190781)
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PurposeExpresses VSV-GS in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190781 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUB6/V5-His
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVSV-GS
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SpeciesSynthetic
- Promoter UbC
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 Fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Synthetic
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUB6-VSV-GS was a gift from Jakob Reiser (Addgene plasmid # 190781 ; http://n2t.net/addgene:190781 ; RRID:Addgene_190781) -
For your References section:
Cell-specific targeting of lentiviral vectors mediated by fusion proteins derived from Sindbis virus, vesicular stomatitis virus, or avian sarcoma/leukosis virus. Zhang XY, Kutner RH, Bialkowska A, Marino MP, Klimstra WB, Reiser J. Retrovirology. 2010 Jan 25;7:3. doi: 10.1186/1742-4690-7-3. 10.1186/1742-4690-7-3 PubMed 20100344