pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)
(Plasmid #190755)

• Purpose: Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.
• Depositing Lab: Dave Piston
• Publication: Foust et al bioRxiv 2021.11.03.467169

Backbone

• Vector backbone: Clontech-style N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 9183
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: GNB1
• Alt name: Gbeta1
• Species: H. sapiens (human)
• GenBank ID: 2782
• Entrez Gene: GNB1 (a.k.a. HG2A, MDS, MRD42)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry2 (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: IRES-R

Gene/Insert 2

• Gene/Insert name: GNG2
• Alt name: Ggamma2
• Species: H. sapiens (human)
• GenBank ID: 54331
• Entrez Gene: GNG2 (a.k.a. HG3F1)
• Tag / Fusion Protein:
  • mCherry2 (N terminal on insert)

Gene/Insert 3

• Gene/Insert name: GNAI1
• Alt name: Galphai1
• Species: H. sapiens (human)
• GenBank ID: 2770
• Entrez Gene: GNAI1 (a.k.a. Gi, HG1B, NEDHISB)
• Tag / Fusion Protein:
  • mEYFP(Q69K)

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: IRES-F

Resource Information

• Supplemental Documents:
  • pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K).gbk
• A portion of this plasmid was derived from a plasmid made by: This plasmid was modified from Addgene #69623.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2021.11.03.467169v1 for bioRxiv preprint.