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Addgene

pmEGFP-ADRA2A
(Plasmid #190753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190753 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+/Hygro
  • Backbone size w/o insert (bp) 5601
  • Total vector size (bp) 8027
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ADRA2A
  • Alt name
    alpha-2A adrenergic receptor
  • Alt name
    alpha-2A AR
  • Species
    H. sapiens (human)
  • GenBank ID
    150
  • Entrez Gene
    ADRA2A (a.k.a. ADRA2, ADRA2R, ADRAR, ALPHA2AAR, ZNF32)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene #24099 and #66216

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmEGFP-ADRA2A was a gift from Dave Piston (Addgene plasmid # 190753 ; http://n2t.net/addgene:190753 ; RRID:Addgene_190753)

  • For your References section:

    Measuring G Protein Activation with Spectrally Resolved Fluorescence Fluctuation Spectroscopy. Foust DJ, Piston DW. bioRxiv 2021.11.03.467169 10.1101/2021.11.03.467169
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