pmEGFP-DRD2
(Plasmid
#190752)
-
PurposeExpression of DRD2 with mEGFP tag on N-terminus.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 190752 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1+/Hygro
- Backbone size w/o insert (bp) 5601
- Total vector size (bp) 7871
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDRD2
-
SpeciesH. sapiens (human)
-
GenBank ID1813
-
Entrez GeneDRD2 (a.k.a. D2DR, D2R)
- Promoter CMV
-
Tag
/ Fusion Protein
- mEGFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byAddgene #24099
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.11.03.467169v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pmEGFP-DRD2 was a gift from Dave Piston (Addgene plasmid # 190752 ; http://n2t.net/addgene:190752 ; RRID:Addgene_190752) -
For your References section:
Measuring G Protein Activation with Spectrally Resolved Fluorescence Fluctuation Spectroscopy. Foust DJ, Piston DW. bioRxiv 2021.11.03.467169 10.1101/2021.11.03.467169