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Addgene

pCD86-mEYFP(Q69K)-mEGFP
(Plasmid #190750)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 6482
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD86
  • Alt name
    B7-2
  • Species
    H. sapiens (human)
  • GenBank ID
    942
  • Entrez Gene
    CD86 (a.k.a. B7-2, B7.2, B70, CD28LG2, LAB72)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mEYFP(Q69K) (C terminal on insert)
    • mEGFP (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene #133858 and #98284

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCD86-mEYFP(Q69K)-mEGFP was a gift from Dave Piston (Addgene plasmid # 190750 ; http://n2t.net/addgene:190750 ; RRID:Addgene_190750)

  • For your References section:

    Measuring G Protein Activation with Spectrally Resolved Fluorescence Fluctuation Spectroscopy. Foust DJ, Piston DW. bioRxiv 2021.11.03.467169 10.1101/2021.11.03.467169
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