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pCDNA3-DCX-TurboID-V5
(Plasmid #190740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone size w/o insert (bp) 5403
  • Total vector size (bp) 7564
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    doublecortin
  • Alt name
    DCX
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_178152
  • Entrez Gene
    DCX (a.k.a. DBCN, DC, LISX, SCLH, XLIS)
  • Promoter CMV
  • Tags / Fusion Proteins
    • TurboID (C terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-DCX-TurboID-V5 was a gift from Jeremy Baskin (Addgene plasmid # 190740 ; http://n2t.net/addgene:190740 ; RRID:Addgene_190740)

  • For your References section:

    Proximity Labeling Reveals Spatial Regulation of the Anaphase-Promoting Complex/Cyclosome by a Microtubule Adaptor. Cao X, Shami Shah A, Sanford EJ, Smolka MB, Baskin JM. ACS Chem Biol. 2022 Aug 11. doi: 10.1021/acschembio.2c00527. 10.1021/acschembio.2c00527 PubMed 35952650
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