pCDNA3-EMTB-TurboID-V5
(Plasmid #190736)

• Purpose: Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
• Depositing Lab: Jeremy Baskin
• Publication: Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021/acschembio.2c00527.

Backbone

• Vector backbone: pCDNA3.1
• Backbone size w/o insert (bp): 5403
• Total vector size (bp): 7306
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ensconsin
• Alt name: EMTB
• Species: H. sapiens (human)
• Insert Size (bp): 816
• Mutation: Microtubule binding-domain of ensconsin (amino acids 18-282 of GenBank reference sequence X73882)
• GenBank ID: X_73882
• Entrez Gene: MAP7 (a.k.a. E-MAP-115, EMAP115)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • TurboID (C terminal on backbone)
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• Supplemental Documents:
  • pCDNA3-EMTB-TurboID-V5.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry