P3-Lenti-dCas9-Tet1-GFP
(Plasmid
#190729)
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PurposeLentiviral construct to express spdCas9-Tet1-GFP.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190729 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFuw-dCas9-Tet1CD
- Total vector size (bp) 16266
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Vector typeMammalian Expression, CRISPR
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Selectable markersZeocin ; EGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namedCas9-tet1
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SpeciesH. sapiens (human)
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MutationChanged Pro434 codon from CCG to CCC and Gly468 from GGA to GGT to remove the BbsI sites
- Promoter UbC
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Tags
/ Fusion Proteins
- SV40 NLS (N terminal on insert)
- T2A-EGFP (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer gcctgtacgagacacggat (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byA portion of this plasmid was derived from a plasmid made by the plasmid derived from Addgene plasmids #84475
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P3-Lenti-dCas9-Tet1-GFP was a gift from Maja Jagodic (Addgene plasmid # 190729 ; http://n2t.net/addgene:190729 ; RRID:Addgene_190729) -
For your References section:
A genetic-epigenetic interplay at 1q21.1 locus underlies CHD1L-mediated vulnerability to primary progressive multiple sclerosis. Pahlevan Kakhki M, Giordano A, Starvaggi Cucuzza C, Venkata S Badam T, Samudyata S, Lemee MV, Stridh P, Gkogka A, Shchetynsky K, Harroud A, Gyllenberg A, Liu Y, Boddul S, James T, Sorosina M, Filippi M, Esposito F, Wermeling F, Gustafsson M, Casaccia P, Hillert J, Olsson T, Kockum I, Sellgren CM, Golzio C, Kular L, Jagodic M. Nat Commun. 2024 Jul 30;15(1):6419. doi: 10.1038/s41467-024-50794-z. 10.1038/s41467-024-50794-z PubMed 39079955