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Addgene

P3-dCas9-Tet1-GFP-Puro
(Plasmid #190728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pdCas9-DNMT3A-EGFP
  • Total vector size (bp) 12965
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin ; EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-tet1
  • Species
    H. sapiens (human)
  • Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 from GGA to GGT to remove the BbsI sites
  • Promoter pCAG
  • Tags / Fusion Proteins
    • 3XFLAG (N terminal on insert)
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer gcctgtacgagacacggat
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from a plasmid made by the plasmid derived from Addgene plasmids #71666 and #84475

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Undesired BbsI restriction sites were removed from the Tet1 sequence, without affecting the amino acid sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P3-dCas9-Tet1-GFP-Puro was a gift from Maja Jagodic (Addgene plasmid # 190728 ; http://n2t.net/addgene:190728 ; RRID:Addgene_190728)

  • For your References section:

    A genetic-epigenetic interplay at 1q21.1 locus underlies CHD1L-mediated vulnerability to primary progressive multiple sclerosis. Pahlevan Kakhki M, Giordano A, Starvaggi Cucuzza C, Venkata S Badam T, Samudyata S, Lemee MV, Stridh P, Gkogka A, Shchetynsky K, Harroud A, Gyllenberg A, Liu Y, Boddul S, James T, Sorosina M, Filippi M, Esposito F, Wermeling F, Gustafsson M, Casaccia P, Hillert J, Olsson T, Kockum I, Sellgren CM, Golzio C, Kular L, Jagodic M. Nat Commun. 2024 Jul 30;15(1):6419. doi: 10.1038/s41467-024-50794-z. 10.1038/s41467-024-50794-z PubMed 39079955
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